To date, most skin studies rely on reconstructed keratinocytes, which do not represent the real skin response.<h4>Objective</h4>Here, we report the distinct effects of IL-4, IL-13, and IL-22 on bio-stabilized human skin with intact barriers and immune cells.<h4>Methods</h4>Spatial transcriptomics on AD-lesions and non-lesional skin was performed. This evidence concerns the gene IL13 and Alzheimer disease.