In our stratified analyses, evaluating age and sex‐specific differences in the associations between the biomarkers and tumor budding, sICAM‐1 was inversely associated with tumor budding among patients with later‐onset colorectal cancer while IL‐8 and sVCAM‐1 were positively associated with tumor budding among patients with early‐onset colorectal cancer. This evidence concerns the gene CXCL8 and neoplasm.