Tissue microarrays from pancreatic cancer patients (n = 44) were classified into two groups by experienced pathologists: 36% of the patients (n = 16) were diagnosed with the severe PNI group, and the others (n = 28) were categorized as the “without PNI group.” We performed IF multi‐staining for CD68 and SRC‐1 in tissue microarrays to evaluate the relationship between SRC‐1‐reprogrammed‐TAMs and PNI in pancreatic cancer. This evidence concerns the gene CD68 and familial pancreatic carcinoma.