Consistently, we observed that Mallory-Denk bodies (MDBs; visualized by immunolabeling for ubiquitin), which are known as reliable histological indices of MASLD severity and have been recently incorporated into the expanded MAS [48], were readily detected in the liver sections from the control groups of mice but not in those from mice lacking STK25 and/or MST3 (Additional File 2: Supplementary Fig. S6). Here, STK25 is linked to metabolic dysfunction-associated steatotic liver disease.