Yoshitake et al. [52] using ST and scRNA-seq technologies in ER + breast cancer, revealing intratumoral heterogeneity and identifying four spatially distinct functional compartments: estrogen-responsive (good prognosis), proliferative (drives growth, poor outcome), hypoxia-induced, and inflammatory—the latter two linked to aggressiveness and therapy resistance. This evidence concerns the gene ESR1 and breast carcinoma.