Further, the impact of 2-OHE2 on the TNF-α signaling pathway was evaluated in RA-FLS, focusing on the inhibition of inflammation, cell proliferation, and apoptosis activation, using Western blotting, quantitative real-time polymerase chain reaction (qRT-PCR), caspase 3/7 activity, scratch assays, and immunocytochemistry. The gene discussed is CASP3; the disease is rheumatoid arthritis.