AKT1 and glioblastoma: To gain a first insight into the signaling cascades involved in the mFUS-mediated effect of inducing the stemness and dormancy properties of residual/peri-focal GBM cells, we performed inhibition experiments in pure, more differentiated GBM cell cultures using the PI3-kinase inhibitor LY294002, followed by Western blot analysis of Akt (Thr308) and GSK3β (Ser9) phosphorylation, along with mRNA expression analysis of dormancy- and stemness-associated genes.