After validation of the successful depletion of the immune cells (Figure 4B, left), a determination of the mRNA expression of dormancy- and stemness-associated markers showed an increased expression of almost all markers to varying degrees (~2-fold and above on average) in the GBM cell-enriched fraction after mFUS (Figure 4B, middle), while only a slight increase of H2BK, Nestin, and SOX2 or no expression (EphA5) was observed in the immune cell-enriched fraction (Figure 4B, right). This evidence concerns the gene EPHA5 and glioblastoma.