Tumor-infiltrating T-cells isolated from both biopsy and CUSA tissue fragment materials were divided into helper (CD4+) and cytotoxic (CD8+) T-cells (Figure 2C) showing similar expression levels of PD-1, CTLA-4, and TIM-3 checkpoint receptors between biopsy and CUSA tissue fragments. This evidence concerns the gene HAVCR2 and neoplasm.