ASZ1 and infection: Effectively, infection efficiency therefore is reduced by the deletion of ORF3 with an infectious titer of 2.0 × 105 FFU/mL (relative infectivity of 4.3 × 103 RNA copies/FFU) compared to an infectious titer of 5.3 × 105 FFU/mL for HEVwt (relative infectivity of 8.8 × 102 RNA copies/FFU).