The results showed that 10 cross-pathway core upregulated hub genes mainly involved in cell cycle/apoptosis regulators: BIRC5, CDC25C, CDKN1C, metabolic/structural genes: DHFR, TTN, PRKAA2, neural/receptor proteins: GABRA1, ADORA1, UCN, tight junction component: CLDN2 and the top enriched pathways were circadian rhythms, apoptosis-multiple species, neuroactive ligand-receptor interaction, antifolate resistance, hypertrophic cardiomyopathy, folate biosynthesis, folate transport and metabolism, morphine addiction, tight junction, and cell cycle. Here, DHFR is linked to hypertrophic cardiomyopathy.