In the present study, fasting blood sugar (105.35±19.48), AST (29.88±24.6), ALT (42.28±17.91), HDL (33.42±13.19), and low-density lipoprotein (LDL) (82.14±40.37) levels were found to be higher in NAFLD patients compared to the control group. The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.