This supports previous work by Martinovich et al. who showed decreased CFTR functional measurements in non-CF HBEb with cryopreservation (Martinovich et al., 2017), but challenges findings reporting unchanged CFTR after cryopreservation in CF HNE and HBEb (Martinovich et al., 2017; Kelly et al., 2022). This evidence concerns the gene CFTR and cystic fibrosis.