In view of the fact that DU145 and PC-3 are human-derived AR-negative prostate cancer cell lines, RM1 is a murine-derived AR-negative prostate cancer cell line, and 22RV1 is an AR-positive prostate cancer cell line, we selected one AR-positive cell line (22RV1) and one AR-negative cell line (RM1) for subsequent experiments. This evidence concerns the gene AR and Familial prostate cancer.