Cellular extract was prepared from several sources including PDFs, spinal cord tissue of mice that were either wildtype or transgenic expressing the SOD1 G93A ALS-causing mutation, and brain tissue from wildtype vs SOD1 G93A mice in the trial shown in Figure 4, including the cohort treated with NCA and SGA-active THIQ compounds. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.