Based on the TCGA database, we first examined PIEZO1 expression in different cancerous and normal tissues, and found PIEZO1 expression was higher in 9 unpaired and paired cancer tissues, including Cholangiocarcinoma (CHOL), Colon Adenocarcinoma (COAD), Esophageal Carcinoma (ESCA), Head and Neck Squamous Cell Carcinoma (HNSC), Kidney Renal Clear Cell Carcinoma (KIRC), Liver Hepatocellular Carcinoma (LIHC), Prostate Adenocarcinoma (PRAD), Rectum Adenocarcinoma (READ), and Stomach Adenocarcinoma (STAD), compared to normal tissues (p < 0.05) (Figures 1A, B). Here, PIEZO1 is linked to head and neck squamous cell carcinoma.