In summary, our findings reveal that under the induction of rEg.P29, miR-378a-5p targeted to BRAF regulation and initiated the differentiation of CD4+T cells in mouse spleen to Th1 direction, and MAPK/ERK pathway may be involved in this process, identifying miR-378a-5p as apotential biomarker and immunomodulatory target in CE. The gene discussed is CD4; the disease is cholesteryl ester measurement.