These include C‐reactive protein (CRP) and orosomucoid protein (A1AG1),[16, 17] well‐established inflammatory markers frequently measured to monitor activity, severity of flare, or response to therapy in inflammatory bowel disease (IBD) with some assays, such as those for CRP, already implemented for routine use in disease monitoring, including IBD.[18, 19, 20] In the current study, we tested the HSP PRM assay using an 11‐point dilution series at four different sample throughputs to find the optimal balance in speed and quantification of all proteins. The gene discussed is ORM1; the disease is inflammatory bowel disease.