TCF7L2 and obesity due to melanocortin 4 receptor deficiency: The MON cluster in our data closely resembles the non-classical monocytes (ncMON) described by Hildreth et al. [26] and Massier et al. [27] in their human datasets, characterized by low expression of CD14 and high expression of FCGR3A and TCF7L2. Prior investigations in humans have described positive associations between this subtype of monocytes and obesity, with adipose tissue from obese patients containing higher amounts of nonclassical monocytes than in adipose tissue from lean patients [66, 67].