Considering these differential p-ERK signals observed with the various ECM types and given that in melanoma cells, MAPK/ERK has recently been shown to negatively control MITF nuclear localization and activity (Estrada et al, 2022; Ngeow et al, 2018), we next asked whether MEK/ERK activation was causal for the observed ECM-dependent MC responses. This evidence concerns the gene MITF and melanoma.