ALB and cystic fibrosis: Calculation of the area under the curve (AUC) to quantify antigen-specific IgG levels (Fig. 1B) revealed statistically significant increases in the levels of tetanus toxoid, Sa Cas9, and Sp Cas9 compared to HSA for non-CF control and CF, although there were no significant differences when comparing non-CF control or CF responses to either Cas9 or the tetanus toxoid positive control.