At MOI 30, Ad5-IκBαΔN and Ad5-GFP infection produced IκBαΔN or GFP protein, respectively, and, whereas Ad5-IκBαΔN inhibited NF-κB reporter activity induced by IL-1β, Ad5-GFP was without effect (Fig. 5, A and B). This evidence concerns the gene IL1B and infection.