CFTR and cystic fibrosis: Bijvelds et al. (2022) established monolayer models using intestinal and biliary organoids derived from CF patients to systematically analyze the impact of CFTR dysfunction on ion transport. Their results showed that the classical CF mutation Phe508del causes impaired Cl− and HCO3− transport, disrupting normal secretory functions in both the intestine and biliary epithelium.