Since the epigenetic silencing occurred at promoter CpG island and was responsible for the downregulation of its expression in primary fibrotic and non-fibrotic cell lines, we explored and confirmed a different profile of SPARC methylation status in primary non-fibrotic (cell line 22) and one-fibrotic cell line (fibrotic UIP, FF24 cell line). This evidence concerns the gene SPARC and idiopathic pulmonary fibrosis.