This study aims to elucidate the role of zinc transporters and the metal-regulatory transcription factor 1 (MTF1)-Notch1-p21 axis in TPEN-induced cell death in human rhabdomyosarcoma (RD) cells, with a focus on cell viability, cell cycle progression, and SOCS3-mediated inflammatory response under zinc-deficient conditions. The gene discussed is SOCS3; the disease is rhabdomyosarcoma.