Interestingly, the trial after 4 weeks of pretreatment with PF (20 mg/kg or 40 mg/kg) reversed the depression behavior of mice, and the abnormal level of inflammatory cytokines in serum, medial prefrontal cortex(mPFC), ventral hippocampus (vHi) and the amygdala, including IL - 6, IL - 1β, TNF-α, IL - 9, IL - 10, IL - 12 and monocyte chemotactic protein 1 (Li et al., 2017). Here, TNF is linked to major depressive disorder.