Additionally, a GEO database search for sequencing depression and stroke-related results revealed that RhoA expression is elevated in depressed mice compared with normal mice (Figure 4A, t = 6.005, df = 36.000, p < 0.0001) (GSE43261), albeit exhibits no difference between patients with IS and normal individuals (Figure 4B, t = 1.085, df = 38.000, p = 0.2847) (GSE22255). The gene discussed is RHOA; the disease is major depressive disorder.