TCAP and hypertrophic cardiomyopathy: If we observe myofibril disarray, it can be a hypertrophic cardiomyopathy (HCM) model though the TCAP's association with HCM has been disputed by the ClinGen in 2022 (https://search.clinicalgenome.org/kb/genes/ HGNC:11610); (5) since we only evaluated the cells that were regularly stimulated, we do not know the contractile function of the cells that showed irregular Ca2+-waves; (6) since the KD cells were derived from a clone cell, they might have been more homogeneous than WT cells.