In addition, when facing patients with suspected bladder cancer or postoperative bladder cancer, we often use cystoscopy coupled with biopsy for screening and diagnosis, but this is an invasive test and not all hospitals can carry out the comparison of urinary HMGB1 expression levels of patients with bladder cancer, patients with urinary tract infections, and healthy people, the expression of urinary HMGB1 in patients with bladder cancer is significantly higher than that of the two, and high-level tumors were also significantly different from lower grades (100). Here, HMGB1 is linked to urinary bladder cancer.