Following the targeting of sgRNA1 and sgRNA2, Ift122 RNA expression was found to be significantly increased in single‐cell clone1 and 2 (p‐value = 0.01, t‐test; Figure 5E) (p‐value = 0.007, t‐test; Figure 5F), which was consistent with the results of the NanoString nCounter assay in NTD fetal samples (Figure 5B and Table S2, Supporting Information). The gene discussed is IFT122; the disease is neural tube defect.