Our results indicated that RAD21 was significantly enriched in the abnormal nervous system with DSBs in both the mBrain and mSpine of NTD mice compared with baseline DSBs in the control group (p‐value < 0.0001, t‐test; Figure 1F,G), indicating that folate deficiency–induced NTD mice burdened with DSBs were associated with disruption of 3D genome organization. The gene discussed is RAD21; the disease is neural tube defect.