To compare the cytotoxicity of DDDs in isogenic cell models we performed CRISPR/Cas9 knock out (KO) of TP53 to generate three isogenic PC models with and without functional TP53: LNCaP (single clone (33)), C4-2B (single clone), and LuCaP 189.4 (KO pool). Here, TP53 is linked to pachyonychia congenita.