To discern between these two fates, we blocked autophagy with chloroquine (CQ), and we observed an increase in the accumulation of LC3-GFP in mIDH1 glioma cells (autophagosomes > 1.6-fold increase in CQ treated mIDH1 cells, P < 0.01; no significance observed in autolysosomes in CQ treated mIDH1 cells vs. CQ treated WT-IDH1 cells) (Figs 8a–j). This evidence concerns the gene IDH1 and central nervous system cancer.