TP53 and central nervous system cancer: To study the impact of autophagy in the context of IDH1R132H mutant gliomas, with TP53 and ATRX inactivating mutations, we previously generated a genetically engineered animal model injecting SB plasmids encoding NRAS G12V, shp53, and shATRX, and with or without IDH1R132H into the lateral ventricle of neonatal mice.15 Sample size and any data inclusion/exclusion were defined individually for each experiment.