The genetically engineered autophagy deficient mouse mIDH1 glioma model was developed using the Sleeping Beauty (SB) Transposon System through a combination of SB-transposase/LUC; NRAS/shP53/GFP; shATRX/GFP; IDH1R132H/Kat and shATG7/CFP plasmids (Fig. 9b). The gene discussed is CFP; the disease is glioma.