Western blot (WB) analysis revealed that in two different mouse cells (NPA/NPAI and RPA/RPAI) and human glioma cells, IDH1 mutations results in increased expression of pULK1-(S555) but decreased expression of pULK1-(S757), when compared with WT-IDH1 glioma cells (Figs. 6b–d; see quantification in Supplementary Fig. 11). Here, RPA1 is linked to central nervous system cancer.