For this study, we used the Sleeping Beauty Transposon system to create three genetically engineered mIDH1 mouse glioma models which include the following genetic lesions: (i) NPA/NPAI: NRAS-G12V, shTP53, shATRX, and IDH1-R132H; and two RAS independent mouse glioma models: (ii) CPA/CPAI: CDKN2A −/−, shTP53, shATRX, and IDH1-R132H; and (iii) RPA/RPAI: PDGFRα-D842V, shTP53, shATRX, and IDH1-R132H. The gene discussed is CDKN2A; the disease is central nervous system cancer.