IDH1 and brain neoplasm: A recent study demonstrated that 2HG can inhibit BCATs, a mechanistic understanding of which identified novel metabolic dependencies in IDH mutant brain tumors.49 To determine whether 2HG influences the metabolic profile in our models of mIDH1 tumor cells, WT-IDH1 NS were cultured with cell-permeable (R)-2HG (O-2HG) for 4h, after which we performed Seahorse extracellular flux analysis.