The induction of sufficient neutrophil infiltration within the tumor microenvironment was achieved through interleukin-8 (IL-8) peritumoral injection combined with NAT, facilitating dynamic observations of tumorigenesis and intratumoral neutrophil dynamics in mice, and the immune role of neutrophils in the tumor microenvironment was explored, highlighting neutrophils with precise navigation as promising effectors for tumor immunotherapy. This evidence concerns the gene CXCL8 and neoplasm.