This study elucidates a novel neutrophil-mediated cytotoxic mechanism through GSDMD–NT–neutrophil extracellular traps (NETs)–NE and demonstrates that combining NAT with peritumoral IL-8 injection effectively inhibits tumor growth, presenting a novel and promising strategy for tumor therapy—“highly selectivity and precise navigation neutrophil therapy”. Here, BRD2 is linked to neoplasm.