TWIST1 and neoplasm: Several lines of evidence support this conclusion: (1) In vivo analysis demonstrated that subcutaneous inoculation of 4T1 cells or administration with SDEVs from tumor-bearing mice increased the expression of Twist1 in the mPFC; (2) Elimination of Twist1 from 4T1 cell decreased Twist1 mRNA and protein levels in the mPFC of recipient mice; (3) Inhibiting EVs synthesis by intraperitoneal injection of GW4869 improves depressive-like behavior in tumor-bearing mice.