ACTA1 and triple-A syndrome: Immunofluorescence staining and Western blot analysis corroborated these findings, showing reduced KIF13B protein expression in human AAA lesions, in which the positive staining content of α-SMA was markedly reduced in the AAA lesions, representing a loss of VSMC, whereas more CD68-positive macrophages were accumulated, indicating an enhanced inflammatory status, compared to adjacent normal aortas (ANAs) (Figure 1C-E).