Blocking R21G03-Gal4 expression in the VNC using tsh-Gal80 (Simpson, 2016) diminished expression of UAS-tdGFP and mutATXN3-(CAG)84 expressed from this driver in the VNC (Fig. 1E; Fig. S1D) and abolished tremor caused by mutATXN3-(CAG)84 (Fig. 1F), demonstrating that its expression in the VNC is necessary for tremor. This evidence concerns the gene LGALS4 and Tremor.