Thus, although blocking chemical transmission via UAS-shibirets (Kitamoto, 2001) within the parameters of our experiment did not cause tremor, hypoactivation of R21G03-Gal neurons with UAS-Kir2.1 and UAS-Hid led to robust tremor, demonstrating that loss of function of these neurons is sufficient to cause tremor. Here, GAL is linked to Tremor.