We next asked whether R21G03-Gal4-driven mutATXN3-(CAG)84 expression in the VNC is sufficient for tremor using tsh-LexA, LexAOp>FLP to selectively remove Tubulin>Gal80 repression only in the VNC [tsh-Gal4 has minimal central brain expression (Simpson, 2016)]. This evidence concerns the gene LGALS4 and Tremor.