By comparison, if an Ovatools risk of 1–2.9% triggered primary care ultrasound and ≥3% triggered urgent cancer pathway referral, 14,803 (11.7%) and 2921 (3.2%) of women ≥50 years and <50 years, respectively, would qualify for further investigation following CA125 (ultrasound or urgent cancer referral). The gene discussed is MUC16; the disease is cancer.