The first loop of Insig1 and Insig2 has been suggested to be responsible for their interactions with PCK1 in 293T cells in an AKT‐dependent manner, specifically requiring PCK1 S90 phosphorylation.[15] Consistently, the expression of Insig1 (Figure 1a, top panel) and Insig2 (Figure 1a, bottom panel) mutants with deletion of the first loop inhibited IGF1 treatment‐enhanced binding of PCK1 to Insig1 and Insig2 in Huh7 human hepatocellular carcinoma (HCC) cells. This evidence concerns the gene INSIG1 and hepatocellular carcinoma.