GCLC and Hepatic fibrosis: Long-term ingestion of APAP can induce liver fibrosis, while andrographolide has been shown to alleviate APAP-induced liver fibrosis in mice by activating Nrf2 and upregulating the expression of downstream genes glutamate-cysteine ligase (GCLC and GCLM) and heme oxygenase-1 (HO-1) (Yan et al., 2018).