To further clarify the role of NINJ1 in AKI, we established three AKI mouse models, including IRI-induced AKI, Cis-induced AKI, and FA-induced AKI (Supplementary Figure 1A-I), and detected a significant increase in NINJ1 expression at both mRNA and protein levels after AKI (Figure 1D, E and Supplementary Figure 1J-N). Here, NINJ1 is linked to acute kidney injury.