Dimensionality reduction and clustering analysis identified 14 cell lineages, including CD4 memory T cells, CD8 T cells, Plasma, Tregs, Epithelial tumor cells, activated B, Macro_Mono, Endothelial Cell, CAFs, T cells, B cells, MEP, Memory B cells, and DC cells (Supplementary Figure 4B). The gene discussed is CD8A; the disease is neoplasm.