HMGB1 and neoplasm: Further assessment demonstrated that the expression of necroptosis-related genes, including FADD, MLKL, TLR2, PGAM, HMGB1, CXCL 1, TRAF2, and EZH2, was elevated in tumor tissue, while FAS, RIPK1, RIPK3, TLR3, TNFRSF, ALDH2, and NDRG2 were upregulated in normal intestinal tissues (Figure 1A).