Clone formation assays further supported this finding: the number of clones formed in HGC-27 and AGS cells significantly decreased from approximately 400 and 300 to less than 100 after PSPC1 knockdown, respectively (****P<0.0001) (Figure 15G), suggesting that PSPC1 has an important role in the long-term proliferative capacity of gastric cancer cells. The gene discussed is PSPC1; the disease is gastric cancer.