Kaplan-Meier survival analysis showed that high PRKAG1 expression in TCGA HCC tissues was negatively correlated with overall survival (OS) and recurrence-free survival (RFS) (both P<0.001), with significantly poorer OS and DFS in the high-expression group compared to the low-expression group (Figures 3G, H). This evidence concerns the gene PRKAG1 and hepatocellular carcinoma.