Comparative analysis indicated that PRKAG1 mRNA was significantly upregulated in various tumor tissues, including breast invasive carcinoma (BRCA), cholangiocarcinoma (CHOL), colon adenocarcinoma (COAD), glioblastoma multiforme (GBM), head and neck squamous cell carcinoma (HNSC), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), lung squamous cell carcinoma (LUSC), and stomach adenocarcinoma (STAD) (all P<0.001) (Figure 3E). Here, PRKAG1 is linked to head and neck squamous cell carcinoma.