PRKAG1 and hepatocellular carcinoma: Western blot analysis showed that PRKAG1 expression in HCC cell lines was significantly higher than that in normal hepatocyte lines (Figures 3I, J); qPCR results confirmed that PRKAG1 mRNA expression levels in MHCC97H and Huh-7 cells were significantly higher than those in normal human hepatocytes (Figure 3K).