Specifically, 19 CpG sites showed strong colocalization with cholelithiasis, including DMPK (cg17499826, cg16686429), LIAS (cg15128470), HEBP1 (cg12479139), PPIF (cg03648155), MRPS21 (cg07843065), SLC25A33 (cg13688474, cg09058554), PRELID1 (cg18824775), RAB24 (cg18824775), DLD (cg17593404), PNKD (cg13835894, cg01329789, cg22712983, cg26786924, cg05991184, cg18259342, cg04880052), and MCCD1 (cg02481669). Here, PPIF is linked to cholelithiasis.