TNF and neoplasm: Therefore, we utilized the double-transgenic zebrafish line Tg(mpeg1:mCherry-F; tnfa:eGFP-F) expressing mCherry in macrophages and eGFP under the control of the tnfa promoter to quantify the M1-like subpopulation of the macrophages within the xenograft tumours (Fig. 5g–i).