Compared to the normal group, the cholestasis model group showed significantly reduced PI3K, p-AKT/AKT, and Bcl-2 expression (P < 0.05, P < 0.01), while GSK-3β, caspase-3, and Bax levels were significantly increased (P < 0.05, P < 0.01, P < 0.001). The gene discussed is GSK3B; the disease is cholestasis.