Following that, the expression of HCAR1 in OS and other sarcoma tissues (including Chondrosarcoma, Ewing sarcoma, and Liposarcoma) was detected using qRT‐PCR and Western blot, and it was found that HCAR1 was significantly up‐regulated in OS tissues as compared to that in other sarcoma tissues (Figure1A–C). This evidence concerns the gene HCAR1 and Ewing sarcoma.