As shown in Table 2, univariate analysis of AL after esophagectomy showed that BMI, high blood pressure, chronic bronchitis, smoking, diabetes, drinking, peptic ulcer, NACT, coagulation, operation way, anesthesia, the lymph node cleaning number, anastomosis way, anastomotic location, postoperative anemia, postoperative albumin, pulmonary infection, and chylothorax were potential risk factors for anastomotic leakage (p < 0.05). The gene discussed is ALB; the disease is anemia (phenotype).