To define the molecular signature of MuSCs in OI versus CTRL conditions, scRNA‐seq was performed on the muscles of 4‐week‐old male rats (two CTRL and two OI), which were sorted (CD45‐, CD31‐ and CD11b‐) to obtain an enrichment in the proportion of MuSCs. This evidence concerns the gene PECAM1 and osteogenesis imperfecta.