In particular, AuNCs, PtNCs, andIrNCs were employed to label specific antibodies to Alpha-Actinin1 (ACTN), Galectin-3-binding protein (LG3BP), and Moesin (MSN), respectively.These three proteins are closely associated with cytoskeletal dynamicsand the early stages of protein aggregation, making them potentiallyrelevant biomarkers in the context of Alzheimer’s disease.The selected proteins are located either partially (MSN) or entirely(LG3BP, ACTN) within the lumen of EVs and, therefore, a lysis protocolwas implemented prior to the immunoassay to expose the EV contentto MNC-labeled antibodies. This evidence concerns the gene MSN and early-onset autosomal dominant Alzheimer disease.